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AWWA WSC70035
- Evaluation of Surface Sampling Techniques for Collection of Bacillus Spores on Pipe Materials
- Conference Proceeding by American Water Works Association, 11/01/2009
- Publisher: AWWA
$12.00$24.00
This powerpoint presentation begins by providing a brief overview of microbial contamination of distribution systems and pipe surface sampling. Defining the problem included the following:studies in measuring contaminant adhesion and decontaminationmust reliably load the pipe surface with a known quantity of contaminant, andmeasure the surface after decontamination to evaluate theeffectiveness of the decontamination;method efficiency reflects the combined losses throughout the process ofsampling and analysis; and,measure contaminant adhesion and decontamination in pilot scale facility. The experimental approach included the following:Bacillus endospores used as contaminant;evaluated pipe surface sampling techniques includedthree types of pipe material (PVC, cement-lined, iron) andtwo sampling techniques (scraping and brushing); and,determined percent adhesion ofthree types of pipe material (PVC, cement-lined, iron). Test protocols are presented, along with a summary of the mass balance approach, a sample recovery comparison, percent adhesion, and relative proportions of spores collected. Presentation conclusions indicate the following: recommended sampling method derived from this study; no one sampling method is optimal for all pipe surfaces; pipematerial and the condition of the surface must be taken into account; and,precision is an important consideration when selecting the samplingtechnique. Includes tables, figures.
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