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AWWA WQTC71353
- Real-time PCR Compared with Culture Method for the Analysis of Legionella in Cooling Towers: A Routine Lab View
- Conference Proceeding by American Water Works Association, 11/01/2009
- Publisher: AWWA
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Theobjectives of this study were to validate a qPCR-based test method and acommercial test kit for the detection and quantification of Legionella spp. and Legionella pneumophila inwater using the AFNOR XP T90-471 method, and to compare qPCR with culture methodNF T90-431 for Legionella testing in 38 cooling tower water samples and 5 hot watersystem samples. In this study, qPCR in conjunction with the commercial test kits used inthe present work were relatively simple to use. However, the detection and quantificationlimits were affected by PCR inhibitors in the DNA extracts from cooling water samples.Therefore, it appears that qPCR cannot become the sole method for Legionella testing offeredby the laboratory, and the culture method remains essential. Includes 9 references, tables, figures.
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