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AWWA WQTC64132
- Selection of Antibiotic Resistant Mutants in a Model Distribution System under Different Disinfection Regimes
- Conference Proceeding by American Water Works Association, 11/01/2006
- Publisher: AWWA
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This slide presentation outlines a study on the selection of antibiotic resistant mutants in a model distribution system under different disinfection regimes. Rationale and research question included:E. coli can enter at distal part of distributionsystem (breaks or pressure transients);if residual disinfectant concentration issublethal and E. coli settles in biofilm, doesselection of resistance to antibiotics occurover time following exposure to disinfectantand or disinfection byproducts (DBPs)? Additional selective pressuresdue to starvation or redox stress may alsobe active. The experimental approach included: spike heterotypic biofilms with E. coli and followthem over time;use wild type strain with known sequence andknown resistance so that mutations can beanalyzed; and,for comparison, use additional strain derived fromwild type strain but which has a known mutationthat fails to repair mutational damage (mismatchrepair mutant; mmr) and confers a mutatorphenotype where mutations accumulate readily. Includes tables, figures.
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