AWWA ACE70572

This powerpoint presentation begins by providing a brief overview of biological perchlorate reductionand molecular biology tools (MBTs) for treatment processes. Research objectives included: develop quantitative assay targetingperchlorate-reduction gene;examine relationship between perchlorateremoval and gene expression levels; and,apply qPCR and RT-qPCR assays toperchlorate treatment bioreactor. Research tasks included:development of quantitative molecular assays forperchlorate treatment; and,application of assays to a bioreactor. Conclusions indicated that: qPCR and RT-qPCR assays provide an additionalline of evidence that microbial perchlorate reductionwas occurring in the bioreactor;cld gene copy numbers generally were higherwhen perchlorate removal was higher;cld gene transcript copy numbers generally werehigher when perchlorate removal was higher; when bioreactor performance declined, genecopies and gene transcript copies also declined; and, bioreactor failure may be explained, at leastin part, by changes in the microbial community. Includes figures.

Product Details

Edition:

Vol. - No.

Published:

11/01/2009

Number of Pages:

25

File Size:

1 file , 1.3 MB